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KMID : 0613820030130050684
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Journal of Life Science
2003 Volume.13 No. 5 p.684 ~ p.691
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Effects of Pine Needle Ethyl Acetate Fraction on Membrane Fluidity and Oxidative Stress in Liver Membranes of Rats
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Choi Jin-Ho
Cho Weon-Ki
Kim Dae-Ik
Baek Seung-Jin
Park Si-Hyang
Kim Nam-Ju
Choi Min-Kyung
Kim Chang-Mok
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Abstract
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This study was designed to investigate the effects of ethyl acetate (EtOAc) fraction of pine (Pinus densiflora Sieb et Zucc) needle extract on membrane fluidity (MF), basal and induced oxygen radical (BOR and IOR), lipid peroxide (LPO) and oxidized protein (OP) as an oxidative stress, and lipofuscin (LF) in liver membranes of male Sprague- Dawley rats. Rats were fed basic diets (control) and experimental diets (EtOAc-25, EtOAc-50 and EtOAc-100) for 45 days. MFs were significantly increased (about 10%) in mitochondria of EtOAc-100 group compared with control group. BOR and IOR formations in mitochondria were significantly inhibited (about 12～18% and 9～12%, respectively) in EtOAc-50 and EtOAc-100 groups, while BOR and IOR formations in microsomes were significantly inhibited (about 9～13% and 18～19%, respectively) compared with control group. LPO levels were significantly inhibited (about 10% and 12～13%, respectively) in mitochondria of EtOAc-100 and microsomes of EtOAc-50 and EtOAc-100 groups, whereas OP levels were significantly inhibited (about 13～14%) in mitochondria of EtOAc-50 and EtOAc-100 groups compared with control group. LF formations were significantly inhibited (about 10～14%) in these three EtOAc groups. These results suggest that ethyl acetate fraction of pine needle may play an effective role in attenuating an oxidative stress and increasing a membrane fluidity.
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KEYWORD
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Pine (Pinus densiflora Sieb et Zucc), Lipid peroxide (LPO), Oxidized protein, Carbonyl group, Membrane fluidity (MF), Lipofuscin (LF)
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